Staining Reagents
Jackson Immuno Research Labs Alexa Fluor 488 AffiniPure F(ab')₂ Fragment Goat Anti-Rabbit IgG, Fc fragment specific, .75mg
Alexa Fluor 488-AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG, Fc Fragment Specific (min X Hu Sr Prot)
Enzo Life Sciences ROS-ID Superoxide detection kit (1Kit)
Enzo Life Sciences’ ROS-ID Superoxide detection kit is designed to directly monitor real time superoxide production in live cells using fluorescence microscopy and/or flow cytometry. A major component of the kit, Superoxide Detection Reagent (Orange), is a cell-permeable probe that reacts specifically with superoxide, generating an orange fluorescent product. The kit is not designed to detect reactive peroxide, hydroxyl, peroxynitrite, chlorine or bromine species, as the fluorescent probe included is relatively insensitive to these analytes. Upon staining, the fluorescent product generated can be visualized using a wide-field fluorescence microscope equipped with a standard orange (e.g., 550/620 nm) fluorescent cube, or cytometrically using any flow cytometer equipped with a blue (488 nm) laser. Applications: Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS .
Vector Laboratories Streptavidin Fluorescein 1 mg
Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have an extremely high affinity for biotin and can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. Fluorescent signals can be easily amplified using biotinylated secondary antibodies followed by fluorochrome-labeled streptavidin or avidin. Fluorescein-labeled streptavidin has an excitation maximum at 495 nm and an emission maximum at 515 nm. Compared to techniques that use only primary and secondary fluorescent antibodies for detection, the use of Fluorescein Streptavidin as an amplifying reagent can provide significantly greater sensitivity and lower background staining.
Sigma Aldrich Fine Chemicals Biosciences BioTracker NucViewR 405 Bl
Caspases are a family of protease enzymes that play essential roles in programmed cell death termed apoptosis. Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9 and sequential activation of plays a central role in the execution-phase of cellular apoptosis.The BioTracker NucView 405 Blue Caspase-3 Dye provides a convenient live cell tool for profiling apoptotic cell populations based on caspase-3/7 activity using either fluorescence microscopy or flow cytometry. In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (fLICA) assays the BioTracker NucView 405 Caspase-3 Dye can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. The substrate consists of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate which is initially non-fluorescent penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells caspase-3/7 cl
Sigma Aldrich Fine Chemicals Biosciences BioTracker Green Copper Li
Copper is one of the trace metals required by living organisms and is an essential cofactor for enzyme activities of cytochrome c oxidase superoxide dismutase and tyrosinase. On the other hand abnormal homeostasis of copper leads to severe disorders such as Menkes syndrome and Wilsons disease or may be related to Alzheimers disease and cancers.BioTracker Green Copper Live Cell Dye is a fluorescent probe to detect Cu which is a dominant redox state of copper in an intracellular reducing environment. The dye is a colorless material with almost no fluorescence. Upon the reaction with Cu its fluorescence intensity at 510 nm increases more than 100 times to that before the reaction. However fluorescence increase is not observed in the presence of reactive oxygen species and other trace metals in cells including Cu2 Mn2 Co2 Ni2 fe fe2 and Zn2.Spectral PropertiesAbsorbance (max) 480nmEmission (max) 510nmResearch CategoryLive Cell Dye
Bioworld Alcian Blue, 100 g
Histological stain for acidic polysaccharides. High Purity Grade EM (610nm, H2O) >26,000
Crescent Chemical Co Inc BROMOPHENOL BLUE-NA-SALT
Bromophenol Blue-Na-salt Tracking dye for electrophoresis; indicator pH 3.4 (yellow) - 4.6 (purple). Size - 5G Storage Conditions - +15 °C TO +30 °C Catalog Number - 15375.01 CAS 34725-61-6
Crescent Chemical Co Inc SERVA BLUE G
SERVA Blue G For preparation of staining solution for protein detection in gel electrophoresis. Size - 25G Storage Conditions - +15 °C TO +30 °C Catalog Number - 35050.02 CAS 6104-58-1